WebAug 21, 2015 · Next, we used CHX (10 μg/ml) to block protein synthesis and then studied hERG recovery in cells precultured in 5 or 0 m m K + medium. ... Western blot analysis revealed that no detectable biotin-labeled hERG channels were present in these cells (Fig. 4 B, right lane), indicating that extracellularly applied sulfo-NHS-SS-biotin does not label ... WebAug 14, 2024 · (A) CHX-dependent hyperphosphorylation of FRQ is impaired in a prd-4 knockout strain. Liquid cultures of WT and ∆prd-4 strains were grown in constant light. Mycelia were harvested before and 2 h after addition of CHX. Western blots were decorated with antibodies against FRQ. (B) PRD-4 is active in extracts from cells …
N6-Methyladenosine-modified lncRNA LINREP promotes …
WebApr 11, 2024 · In Western blot analysis with p53S367p antibody, high levels of p53S367p were detected in undamaged control cells, but its marked decrease was evident 24 h after etoposide/doxorubicin treatment ... WebJun 7, 2024 · Western blot analysis of PD-L1 expression. J H1299 cells were transiently … camping at portland international raceway
Puromycin labeling does not allow protein synthesis to be
WebCell division cycle 25A (Cdc25A) is a dual-specificity phosphatase that is overexpressed in several cancer cells and promotes tumorigenesis. In normal cells, Cdc25A expression is regulated tightly, but the changes in expression patterns in cancer cells that lead to tumorigenesis are unknown. In this study, we showed that ubiquitin-specific protease 29 … WebOct 4, 2024 · We investigated this mechanism further by treating wild-type (WT) and COX7A2 KO cells for up to 24 h with the cytosolic protein translation inhibitor cycloheximide (CHX). Western blotting of mitochondrial lysates ( Figure 1 C) showed a steady decline of SCAFI levels in WT cells, but unaltered high levels of this protein in COX7A2 KO cells ... WebSep 17, 2010 · Cycloheximide (CHX, Sigma-Aldrich, Inc.), an inhibitor of protein synthesis in eukaryotic organisms, was added to the medium 36 hrs after transfection at 50 ug/ml final concentration. The cell lysates were collected at 0, 30, 60 and 120 mins after addition of CHX. Western blot analysis was performed as describe above. first volunteer bank ringgold ga