Granuphilin c2a

Author: dsqf

August undefined, 2024

WebLearn about Equinix DC2 carrier-neutral data center, located at 21715 Filigree Court, Ashburn, VA. See our interconnection options, certifications and more. Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the …

Pancreatic β-Cell Protein Granuphilin Binds Rab3 and Munc-18 …

WebCorvallis Veterinary Hospital. Jul 2007 - Sep 20092 years 3 months. 1543 NW 9th St. Corvallis, OR 97330. As part of a small veterinary clinic I gained proficiency in a diverse set of skills ... Webusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding afﬁnity of this protein. This suggests that granuphilin may serve as a coincidence de- diamond airport parking lot a

SYTL4 (synaptotagmin like 4)

WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. WebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … WebJan 1, 2024 · The C2A domain of Slp-4 is known to drive this protein's ability to bind cellular PMs primarily via interaction with PIP 2 (12, 13).However, the protein retains an affinity for membranes containing the anionic lipids PS and/or PI, even in the absence of PIP 2 ().Here, we set out to discern the contributions of PIP 2 and background anionic lipid binding to … diamond airport parking discount code

Multivalent lipid targeting by the calcium-independent C2A …

Here

WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. Slp-4 binds vesicular Rab proteins via an N-terminal … WebAug 24, 2024 · The C2A domain of granuphilin binds strongly to plasma membranes containing PIP2 via a conserved lysine cluster (red circle) embedded in a large electropositive surface (blue oval) containing many basic residues (blue sticks). A single exposed hydrophobic residue (gray sticks) also contributes to membrane affinity. circle insert cushionWebJun 3, 2024 · Synaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before … circle inscribed in a right angled triangle

"WebThe C2A domain plays key roles in this process through interaction with PI (4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A … " - Granuphilin c2a

Granuphilin c2a

WebC2 Education is the fastest-growing test prep and tutoring center in the country. Use our Center Locator to help find a center near you and get started! WebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma membrane cytosolic leaflet (Corbin et al., 2007, Voelker, 2008).Since some C2 domains are known to dock preferentially to PIP x lipids, such physiologically relevant lipid mixtures …

Did you know?

WebJan 27, 2015 · Granuphilin C2A has similar affinity for liposomes containing either 24% PS or 2% PIP2, but affinity increases ∼100-fold in the presence of both target lipids. Binding site(s) for the two lipids ... WebGranuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this …

WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … WebJan 1, 2024 · The results of an in vitro binding study indicated that the C2A domain of Slp3 and Slp5 functions as an atypical Ca 2+-dependent phospholipid-binding domain ... Slp4/granuphilin was originally identified as a rabphilin-like protein associated with insulin granules in pancreatic β cells (Wang et al. 1999).

WebGranuphilin C2A displays a lateral diffusion constant comparable to other C2 domains. However, the diffusion of the granuphilin C2AB tandem on the same membrane appears to be slower; suggesting substantial PS contacts for the C2B domain within the C2AB tandem. This effect represents a weak WebGranuphilin is a membrane binding protein responsible, in part, for docking insulin secretory vesicles to the plasma membrane. Granuphilin, also known as synaptotagmin …

WebMay 29, 2024 · Multivalent lipid targeting by the calcium-independent C2A domain of Slp-4/granuphilin Aml A Alnaas 1, Abena Watson-Siriboe 1, Sherleen Tran , Mikias Negussie, Jack A. Henderson , J. Ryan Osterberg 1, Nara Lee Chon , Julianna Oviedo,1 Tatyana Lyakhova1, Cole Michel2, Nichole Reisdorph 2, Richard Reisdorph , Colin T. Shearn3, … diamond airport storage anchorageWebNov 5, 1990 · Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin granules onto plasma membrane. The C2A domain plays key roles in this process through interaction with PI(4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A domain to PI(4,5)P2 head group, and further … circle inscribed in a triangle theoremNMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. diamond air purifierWeband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its circle inscribed in right angle triangleWebApr 1, 2016 · Furthermore, granuphilin forms a 180-nm cluster at the site of each docked granule, along with granuphilin-interacting Rab27a and Munc18-1 clusters. These … circle inscribed in isosceles triangleWebFeb 11, 2024 · Seventy percent of the world’s internet traffic passes through all of that fiber. That’s why Ashburn is known as Data Center Alley. The Silicon Valley of the east. The … circle in sectionsWebSep 1, 2014 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P 2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. circle insects worksheet